Cellulase assays.
نویسندگان
چکیده
Cellulose is a heterogeneous polysaccharide, and its enzymatic hydrolysis requires endoglucanase, exoglucanase (cellobiohydrolase), and beta-glucosidase to work together. We summarize the most commonly used assays for individual enzymes and cellulase mixture.
منابع مشابه
Cellulase activities in biomass conversion: measurement methods and comparison.
Cellulose, the major constituent of all plant materials and the most abundant organic molecule on the Earth, is a linear biopolymer of glucose molecules, connected by β-1,4-glycosidic bonds. Enzymatic hydrolysis of cellulose requires mixtures of hydrolytic enzymes including endoglucanases, exoglucanases (cellobiohydrolases), and β-glucosidases acting in a synergistic manner. In biopolymer hydro...
متن کاملCellulase Activities in Biomass Conversion: Measurement Methods
16 Cellulose, the major constituent of all plant materials and the most abundant 17 organic molecule on the Earth, is a linear biopolymer of glucose molecules, connected 18 by β-1,4-glycosidic bonds. Enzymatic hydrolysis of cellulose requires mixtures of 19 hydrolytic enzymes including endoglucanases, exoglucanases (cellobiohydrolases) and 20 β-glucosidases acting in a synergistic manner. In bi...
متن کاملElectrical lysis of cells for detergent-free droplet assays.
Efficient lysis is critical when analyzing single cells in microfluidic droplets, but existing methods utilize detergents that can interfere with the assays to be performed. We demonstrate robust cell lysis without the use of detergents or other chemicals. In our method, cells are exposed to electric field immediately before encapsulation in droplets, resulting in cell lysis. We characterize ly...
متن کاملBiotechnology for Biofuels Commentary Measurement of saccharifying cellulase
This article sets forth a simple cellulase assay procedure. Cellulose is variable in nature, insoluble and resistant to enzymatic attack. As a result there have been a bevy of bewildering cellulase assays published that yielded irrational results. Certain protocols focused on the rapidity of the assay while ignoring that only the most readily susceptible cellulose regions were being hydrolyzed....
متن کاملMeasurement of saccharifying cellulase
This article sets forth a simple cellulase assay procedure. Cellulose is variable in nature, insoluble and resistant to enzymatic attack. As a result there have been a bevy of bewildering cellulase assays published that yielded irrational results. Certain protocols focused on the rapidity of the assay while ignoring that only the most readily susceptible cellulose regions were being hydrolyzed....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Methods in molecular biology
دوره 581 شماره
صفحات -
تاریخ انتشار 2009